表征两个不同工作范围的双感测系统电感式生物传感器

a)

b)

图17 a)描述了传感器7B与工作范围呈函数关系的行为特性；b) 图17的放大图，突出显示中间带。

4.结论

本文介绍了一个电感式生物传感器。该传感器能够将特定生物识别事件转换成电信号并输出，这里的生物识别事件是通过一个涉及使用磁性标记物的过程，来识别分析物（抗原或DNA序列）与其特定识别元件（抗体或寡核苷酸)之间发生的特定生物事件。因为热致动和感测的存在，传感器可用于多种生物识别应用，例如，DNA杂交、蛋白质测量等。设计优化和两个不同的感测子系统（小线圈和大线圈）使该传感器取得广泛的动态范围，强化了生物应用功能。下一步工作是提高传感器的响应性，在线圈区的衬底背后沉积磁层，以此提高传感器的敏感度。

参考文献

[1]K. Larsson, K. Kriz, D. Kriz, Magnetic transducers in biosensors and bioassays, Analysis 27 (1999) 617–621

[2]I-M. Hsing, Y. Xu, W. Zhao, Micro- and Nano- Magnetic Particles for Applications in Biosensing, Electroanalysis, Vol. 19, Issue 7-8, pp. 755-768, 2007

[3]G.M.Brett, S.J.Chambers, L. Huang, M.R.A.Morgan, Design development of immunoassays for detection of proteins, Food Control 10 (6) (1999) 401–406

[4]Rife, J.C., Miller, M.M., Sheehan, P.E., Tamanaha, C.R., Tondra, M., and Whitman, L.J.:‘Design and performance of GMR sensors for the detection of magnetic microbeads in biosensors’, Sens.Actuat.A, Phys., 2003, 107, (3), pp. 209–218

[5]McKendry, R., Zhang, J., Arntz, Y., Strunz, T., Heger, M., and Lang, H.P.:‘Multiple label free biodetection and qunatitative DNA-binding assays on a nanomechanical cantilever array’, Proc.Natl.Acad.Sci.USA, 2002, 99, pp. 9783–9788

[6]Enpuku, K., Hotta, M., and Nakahodo, A.:‘High-Tc SQUID system for biological immunoassays’, Phys.C:Supercond., 2001, 357–360, (Part 2), pp. 1462–1465

[7]Ferreira, H.A., Graham, D.L., Freitas, P.P., Cabral, J.M.S., 2003.J. Appl.Phys.93, 7281–7286

[8]Ejsing, L., Hansen, M.F., Menon, A.K., Ferreira, H.A., Graham, D.L., Freitas, P.P., 2004.Appl.Phys.Lett.84, 4729-4731

[9]Ludwig, F., Heim, E., Maeuselein, S., Eberbeck, D., Schilling, M., 2005.J.Magn.Magn.Mater.293, 690-695

[10]Andò, B., Ascia, A., Baglio, S., Bulsara, A.R., In, V., Pitrone, N., Trigona, C., Residence times difference fluxgate magnetometer for magnetic biosensing, (2008) AIP Conference Proceedings, 1025, pp. 139-149.

[11]Andò, B., Baglio, S., Pitrone, N., Trigona, C., Bulsara, A.R., In, V., Coltelli, M., Scollo, S., A novel measurement strategy for volcanic ash fallout estimation based on RTD Fluxgate magnetometers (2008) Conference Record - IEEE Instrumentation and Measurement Technology Conference, art. no. 4547358, p. 1904-1907

[12]J-W. Choi, , T. M. Liakopoulos, C. H. Ahn, An on-chip magnetic bead separator using spiral electromagnets with semi-encapsulated permalloy, Biosensors and Bioelectronics, Vol. 16, Issue 6, August 2001, Pages 409–416

[13]C. Serre, S. Martìnez, A. Pérez-Rodrìguez, J. R. Morante, J. Esteve, J. Montserrat, Si technology based microinductive devices for biodetection applications, Sensors and Actuators A:Physical, Vol. 132, Issue 2, 20 November 2006, Pages 499–505

[14]S. Martínez, F. X. Muñoz, E. Baldrich, Inductive microcoils for the fast and simple detection of bacterial presence, Sensors and Actuators B:Chemical, Vol. 147, Issue 1, 18 May 2010, Pages 304–309

[15]M. Cortés, S. Martínez, C. Serre, E. Gómez, A. Pérez-Rodríguez, E. Vallés, Design and electrochemical preparation of inductive copper coils for magnetic particles detection, Sensors and Actuators B:Chemical, Vol. 173, October 2012, Pages 737–744

[16]J. Eveness, J.Kiely, P. Hawkins, P. Wraith, R.Luxton, Evaluation of paramagnetic particles for use in a resonant coil magnetometerbased magneto-immunoassay, Sensors and Actuators B:Chemical, Vol. 139, Issue 2, 4 June 2009, Pages 538–542

[17]P. Hawkins, R. Luxton, J. Macfarlane, Measuring system for the rapid determination of the concentration of coated micrometer-sized paramagnetic particles suspended in aqueous buffer solutions, Rev. Sci.Instrum.Vol.72, Issue 1, 237-242, (2001)

[18]J. Kiely, P. Hawkins, P. Wraith and R. Luxton, Paramagnetic particle detection for use with an immunoassay based biosensor, Science, Measurement & Technology, IET, Vol.1, Issue 5, 270 – 275, 2007

[19]C.B.Kriz, K. Radevik, D. Kriz, "Magnetic permeability measurements in bioanalysis and biosensors, Analytical chemistry, Vol. 68, Issue 11, 1966-1970, 1996

[20]M. H.F.Meyer, M. Stehr, S. Bhuju, H.J. Krause, M. Hartmann, P. Miethe, M. Singh, M. Keusgen, Magnetic biosensor for the detection of Yersinia pestis, Journal of Microbiological Methods,Vol.68, issue 2, 218–224, 2007

[21]S. Baglio, S. Castorina,N.Savalli, Integrated inductive sensors for the detection of magnetic microparticles, Sensors Journal IEEE, Vol. 5, Issue 3, 372 – 384, 2005

[22]B. Andò, S. Baglio, A. Beninato, G. Fallica, V.Marletta, N.Pitrone, Analysis and design of inductive biosensors for magnetic immuno assay, 19th IMEKO World Congress 2009, Volume 4, 2009, Pages 2128-2132

[23]M. E. Castagna, S. Petralia, M. G. Amore, E. Cappello, A. Beninato, V.Sinatra, S. Baglio, S. Conoci, A novel silicon based mags-biosensor for nucleic acid detection by magnetoelectronic transduction, EMRS conference, accepted